Controlling the nitrogen environment for optimal Rhodomonas salina production

Abstract The microalga Rhodomonas salina is a widely used species for rearing live feed organisms in the aquaculture market. A species-specific medium an essential step towards enhancing productivity and decreasing production costs microalgae cultivation. However, relevant aspects of composition such as nitrogen source elemental ratio have not yet been characterized this alga. This study aimed to optimize following three culture media: 1) optimal between phosphorus (N:P ratio); 2) preferred nitrogen; 3) tolerance R. free ammonia. To investigate this, we conducted series controlled laboratory experiments shake flasks. Our revealed 45% increase growth rate when N:P 15:1 was compared standard 25:1. Ammonium nitrate were equally well accepted source, however, mix ammonium resulted significant reduction. Free ammonia did affect alga at tested concentrations up 5 mg ammonia–nitrogen L −1 . We conclude that cultivation, strongly preferred, it leads rate. Further, media with single promote faster over mixed sources, may safely be since tolerates certain levels Overall, work provides insights into cultivation conditions , allowing more efficient reliable species.